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c rabbit anti p2x2  (Alomone Labs)


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    Structured Review

    Alomone Labs c rabbit anti p2x2
    C Rabbit Anti P2x2, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 94/100, based on 36 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/c+rabbit+anti+p2x2/Anti-P2X3+Receptor+Antibody/pm41016735-108-64-68
    Average 94 stars, based on 36 article reviews
    c rabbit anti p2x2 - by Bioz Stars, 2026-09
    94/100 stars

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    Isolation:

    Article Title: Optochemical modulation of corneal cold nerve terminal impulse activity with a photochromic ion channel blocker.
    Article Snippet: .. The corneas were isolated, cut into quadrants and incubated for 2 h at room temperature in a blocking solution containing 5% normal goat serum (Vector Laboratories, Burlingame, CA, USA), 1% bovine serum albumin (Vector Laboratories) and 0.1% Triton X-100 (Sigma-Aldrich) at room temperature for 2 h. Subsequently, the corneas were incubated with primary antibodies diluted in the blocking solution for 4 days at 4 C. Rabbit anti-P2X2 (1:50; Alomone Labs Cat# APR-016, RRID:AB_2313760) and anti-P2X3 (1:50; Alomone Labs Cat# APR-003, RRID:AB_2040054), mouse anti-neuronal class III β-tubulin (1:500; Covance Cat# MMS-435P, RRID:AB_2313773) and chicken anti-GFP (1:500; Abcam Cat# ab13970, RRID:AB_300798) were utilized. .. After rinsing with PBS, corneas were incubated with secondary antibodies Alexa Fluor 488 goat anti-chicken IgG (1:500; Molecular Probes Cat# A-11039, RRID:AB_142924), Alexa Fluor 555 goat anti-mouse IgG (1:500; Thermo Fisher Scientific Cat# A32727, RRID:AB_2633276) and Alexa Fluor 647 goat anti-rabbit IgG (1:500; Thermo Fisher Scientific Cat# A32733, RRID:AB_ 2633282) in PBS for 2 h at room temperature, followed by incubation with Hoechst 33342 at 10 μg ml 1 (Thermo Fisher Scientific, Waltham, MA, USA) for 10 min at room temperature.

    Incubation:

    Article Title: Optochemical modulation of corneal cold nerve terminal impulse activity with a photochromic ion channel blocker.
    Article Snippet: .. The corneas were isolated, cut into quadrants and incubated for 2 h at room temperature in a blocking solution containing 5% normal goat serum (Vector Laboratories, Burlingame, CA, USA), 1% bovine serum albumin (Vector Laboratories) and 0.1% Triton X-100 (Sigma-Aldrich) at room temperature for 2 h. Subsequently, the corneas were incubated with primary antibodies diluted in the blocking solution for 4 days at 4 C. Rabbit anti-P2X2 (1:50; Alomone Labs Cat# APR-016, RRID:AB_2313760) and anti-P2X3 (1:50; Alomone Labs Cat# APR-003, RRID:AB_2040054), mouse anti-neuronal class III β-tubulin (1:500; Covance Cat# MMS-435P, RRID:AB_2313773) and chicken anti-GFP (1:500; Abcam Cat# ab13970, RRID:AB_300798) were utilized. .. After rinsing with PBS, corneas were incubated with secondary antibodies Alexa Fluor 488 goat anti-chicken IgG (1:500; Molecular Probes Cat# A-11039, RRID:AB_142924), Alexa Fluor 555 goat anti-mouse IgG (1:500; Thermo Fisher Scientific Cat# A32727, RRID:AB_2633276) and Alexa Fluor 647 goat anti-rabbit IgG (1:500; Thermo Fisher Scientific Cat# A32733, RRID:AB_ 2633282) in PBS for 2 h at room temperature, followed by incubation with Hoechst 33342 at 10 μg ml 1 (Thermo Fisher Scientific, Waltham, MA, USA) for 10 min at room temperature.

    Blocking Assay:

    Article Title: Optochemical modulation of corneal cold nerve terminal impulse activity with a photochromic ion channel blocker.
    Article Snippet: .. The corneas were isolated, cut into quadrants and incubated for 2 h at room temperature in a blocking solution containing 5% normal goat serum (Vector Laboratories, Burlingame, CA, USA), 1% bovine serum albumin (Vector Laboratories) and 0.1% Triton X-100 (Sigma-Aldrich) at room temperature for 2 h. Subsequently, the corneas were incubated with primary antibodies diluted in the blocking solution for 4 days at 4 C. Rabbit anti-P2X2 (1:50; Alomone Labs Cat# APR-016, RRID:AB_2313760) and anti-P2X3 (1:50; Alomone Labs Cat# APR-003, RRID:AB_2040054), mouse anti-neuronal class III β-tubulin (1:500; Covance Cat# MMS-435P, RRID:AB_2313773) and chicken anti-GFP (1:500; Abcam Cat# ab13970, RRID:AB_300798) were utilized. .. After rinsing with PBS, corneas were incubated with secondary antibodies Alexa Fluor 488 goat anti-chicken IgG (1:500; Molecular Probes Cat# A-11039, RRID:AB_142924), Alexa Fluor 555 goat anti-mouse IgG (1:500; Thermo Fisher Scientific Cat# A32727, RRID:AB_2633276) and Alexa Fluor 647 goat anti-rabbit IgG (1:500; Thermo Fisher Scientific Cat# A32733, RRID:AB_ 2633282) in PBS for 2 h at room temperature, followed by incubation with Hoechst 33342 at 10 μg ml 1 (Thermo Fisher Scientific, Waltham, MA, USA) for 10 min at room temperature.



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